Part:BBa_K3819003:Design

EGL7 (encode cellulase from Aspergillus fumigatus Af293)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.

Source

EGL7 (encode cellulase from Aspergillus fumigatus Af293)

References

1 Vianna Bernardi A, Kimie Yonamine D, Akira Uyemura S, Magnani Dinamarco T. A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis. Int J Mol Sci. 2019;20(9):2261. Published 2019 May 7. doi:10.3390/ijms20092261 2 Bernardi, A.V., De Gouvêa,P.F., Gerolamo, L.E., Yonamine, D.K., De Lourdes De Lima Balico,L., Uyemura, S.A., Dinamarco, T.M., 2018. Functional characterization of GH7 endo-1,4-β-glucanase from Aspergillus fumigatus and its potential industrial application. Protein Expression and Purification..doi:10.1016/j.pep.2018.04.016